The Food and Agriculture Organization of the United Nations (FAO) launched an initiative to assess the existing impediments for scaling innovation and technology in food and agriculture (AgTech) and to identify options to improve the enabling environment for AgTech-focused businesses. The FAO tasked the Yield Lab Institute with building a framework tool for this important AgTech initiative. The initiative offers a tool for decision makers to promote the uptake of AgTech, investment and entrepreneurship in Africa, ultimately to advance agricultural productivity and food security. The first cohort evaluates the AgTech ecosystems in three East African countries: Kenya, Rwanda, and Uganda.